Question:
What does the analysis involve?

The samples are processed as follows:

• The DNA is extracted from the sample
• The DNA extract is tested for PCR inhibition using a real-time PCR assay. If inhibition is found the sample is diluted prior to testing for the presence of great crested newts
• The sample is tested for degradation using a real-time PCR assay
• The sample is tested for presence of GCN DNA using a real-time PCR assay